Difference between revisions of "Part:BBa K3038000"

Line 3: Line 3:
 
<partinfo>BBa_K3038000 short</partinfo>
 
<partinfo>BBa_K3038000 short</partinfo>
  
ADR : GenBank: P42327; https://www.ncbi.nlm.nih.gov/protein/P42327  
+
===ADR : GenBank: P42327===
Alcohol dehydrogenase; Short=ADH
+
https://www.ncbi.nlm.nih.gov/protein/P42327  
 +
===Alcohol dehydrogenase; Short=ADH===
  
 
Thermophilic NAD+-dependent alcohol dehydrogenase. Bears mainly an ethanol-dehydrogenase activity.
 
Thermophilic NAD+-dependent alcohol dehydrogenase. Bears mainly an ethanol-dehydrogenase activity.
  
reaction :a secondary alcohol + NAD+ = a ketone + H+ + NADH
+
===reaction=== :a secondary alcohol + NAD+ = a ketone + H+ + NADH
 
EC:1.1.1.1
 
EC:1.1.1.1
  
PROTEIN SEQUENCE    
+
===PROTEIN SEQUENCE ===   
 
MHHHHHHKAAVVNEFKKALEIKEVERPKLEEGEVLVKIEACGVCHTDLHAAHGDWPIKPKLPLIPGHEGVGIVVEVAKGVKS
 
MHHHHHHKAAVVNEFKKALEIKEVERPKLEEGEVLVKIEACGVCHTDLHAAHGDWPIKPKLPLIPGHEGVGIVVEVAKGVKS
 
IKVGDRVGIPWLYSACGECEYCLTGQETLCPHQLNGGYSVDGGYAEYCKAPADYVAKIPDNLDPVEVAPILCAGVTTYKALK
 
IKVGDRVGIPWLYSACGECEYCLTGQETLCPHQLNGGYSVDGGYAEYCKAPADYVAKIPDNLDPVEVAPILCAGVTTYKALK

Revision as of 09:43, 10 October 2019


Alcohol dehydrogenase - Geobacillus stearothermophilus cMYC and 6His N-terminal tags

ADR : GenBank: P42327

https://www.ncbi.nlm.nih.gov/protein/P42327

Alcohol dehydrogenase; Short=ADH

Thermophilic NAD+-dependent alcohol dehydrogenase. Bears mainly an ethanol-dehydrogenase activity.

===reaction=== :a secondary alcohol + NAD+ = a ketone + H+ + NADH EC:1.1.1.1

PROTEIN SEQUENCE

MHHHHHHKAAVVNEFKKALEIKEVERPKLEEGEVLVKIEACGVCHTDLHAAHGDWPIKPKLPLIPGHEGVGIVVEVAKGVKS IKVGDRVGIPWLYSACGECEYCLTGQETLCPHQLNGGYSVDGGYAEYCKAPADYVAKIPDNLDPVEVAPILCAGVTTYKALK VSGARPGEWVAIYGIGGLGHIALQYAKAMGLNVVAVDISDEKSKLAKDLGADIAINGLKEDPVKAIHDQVGGVHAAISVAVN KKAFEQAYQSVKRGGTLVVVGLPNADLPIPIFDTVLNGVSVKGSIVGTRKDMQEALDFAARGKVRPIVETAELEEINEVFER MEKGKINGRIVLKLKED



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]