Difference between revisions of "Part:BBa K2938015"
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The plasmid was constructed using gibson assembly, then was incorporated into E.coli BL21 and DH5a using Heat shock, and into serratia marcescens 274 using electroporation. | The plasmid was constructed using gibson assembly, then was incorporated into E.coli BL21 and DH5a using Heat shock, and into serratia marcescens 274 using electroporation. | ||
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| − | + | ===Characterization=== | |
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| + | Western of Cry11Aa subunit with anti-Strep antibody. proving the translation of the toxin subunit on the plasmid. | ||
| − | + | and confocal microscope photo showing our transgenic bacteria presence in the mosquitoes larva | |
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| − | + | [[File:Larva GFP.png|200px|thumb|left|Mosquitoes' larva that was fed with serratia marcescens 274 expressing plasmid with Cry11Aa and deGFP. confocal microscope (x10)]] | |
| − | + | [[File:Cry11Aa_Western.png|200px|thumb|right|Western Blot of Bacteria expressing Cry11Aa and deGFP – proof of Cry11Aa expression]] | |
Revision as of 10:03, 12 October 2019
Cry11Aa (Strep tag) + deGFP Device
PR1 - RBS - Cry11Aa(Strep tag) - RBS - deGFP - T500 Terminator
This plasmid was planned on a pBEST plasmid backbone. It contains larvicidal activity, as it containing toxic subunit of the bacillus thuringiensis israelensis (BTI) pBtoxis plasmid which is toxic to mosquitoes larva.
The plasmid was constructed using gibson assembly, then was incorporated into E.coli BL21 and DH5a using Heat shock, and into serratia marcescens 274 using electroporation.
Characterization
Western of Cry11Aa subunit with anti-Strep antibody. proving the translation of the toxin subunit on the plasmid.
and confocal microscope photo showing our transgenic bacteria presence in the mosquitoes larva
