Difference between revisions of "Part:BBa K3038001:Experience"

(→‎Applications of BBa_K3038001)
(→‎Applications of BBa_K3038001)
Line 17: Line 17:
 
Modelization of the ligation between ADR N-ter/pSB1A3 and ADR C-ter/pSB1A3 with Geneious software. This map shows the pBAD promoter and its terminator flanking the coding sequence of the ADR protein. Also present in N-ter or C-ter are 6-His and c-myc tag. Finally, in the plasmid is present and ampicillin resistance cassette.
 
Modelization of the ligation between ADR N-ter/pSB1A3 and ADR C-ter/pSB1A3 with Geneious software. This map shows the pBAD promoter and its terminator flanking the coding sequence of the ADR protein. Also present in N-ter or C-ter are 6-His and c-myc tag. Finally, in the plasmid is present and ampicillin resistance cassette.
  
−
[[Image:T--Poitiers--plasmid_construction_ADR-tab3.jpg|thumb|right|300px|'''Figure 1.  Single stranded DNA harvested from phage and run on a gel.  The higher bands are phage genomic DNA while the lower bands at roughly 2-3kb correspond to the expected plasmid size.''']]
+
[[Image:plasmid_construction_ADR-tab3.jpg|thumb|right|300px|'''Figure 1.  Single stranded DNA harvested from phage and run on a gel.  The higher bands are phage genomic DNA while the lower bands at roughly 2-3kb correspond to the expected plasmid size.''']]
  
 
===User Reviews===
 
===User Reviews===

Revision as of 09:12, 10 October 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3038001

Characterization of the ADR constructs:


Electrophoresis gel photography following deposits of N-ter and C-ter ADR PCR products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is NEB 1 kb Plus DNA Ladder.



Modelization of the ligation between ADR N-ter/pSB1A3 and ADR C-ter/pSB1A3 with Geneious software. This map shows the pBAD promoter and its terminator flanking the coding sequence of the ADR protein. Also present in N-ter or C-ter are 6-His and c-myc tag. Finally, in the plasmid is present and ampicillin resistance cassette.

File:Plasmid construction ADR-tab3.jpg
Figure 1. Single stranded DNA harvested from phage and run on a gel. The higher bands are phage genomic DNA while the lower bands at roughly 2-3kb correspond to the expected plasmid size.

User Reviews

UNIQ2ec7e443b94bb33c-partinfo-00000000-QINU UNIQ2ec7e443b94bb33c-partinfo-00000001-QINU