Difference between revisions of "Part:BBa K3038001:Experience"

Revision as of 09:11, 10 October 2019

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Applications of BBa_K3038001

Characterization of the ADR constructs:

Electrophoresis gel photography following deposits of N-ter and C-ter ADR PCR products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is NEB 1 kb Plus DNA Ladder.

Modelization of the ligation between ADR N-ter/pSB1A3 and ADR C-ter/pSB1A3 with Geneious software. This map shows the pBAD promoter and its terminator flanking the coding sequence of the ADR protein. Also present in N-ter or C-ter are 6-His and c-myc tag. Finally, in the plasmid is present and ampicillin resistance cassette.

Figure 1. Single stranded DNA harvested from phage and run on a gel. The higher bands are phage genomic DNA while the lower bands at roughly 2-3kb correspond to the expected plasmid size.

User Reviews

UNIQ379b01af12237ee7-partinfo-00000000-QINU UNIQ379b01af12237ee7-partinfo-00000001-QINU

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 Modelization of the ligation between ADR N-ter/pSB1A3 and ADR C-ter/pSB1A3 with Geneious software. This map shows the pBAD promoter and its terminator flanking the coding sequence of the ADR protein. Also present in N-ter or C-ter are 6-His and c-myc tag. Finally, in the plasmid is present and ampicillin resistance cassette.
+[[Image:Washington_f1_origin_gel.png|thumb|right|300px|'''Figure 1.  Single stranded DNA harvested from phage and run on a gel.  The higher bands are phage genomic DNA while the lower bands at roughly 2-3kb correspond to the expected plasmid size.''']]
 ===User Reviews===