Difference between revisions of "Part:BBa K3038000:Experience"
(→Applications of BBa_K3038000) |
(→Applications of BBa_K3038000) |
||
| Line 28: | Line 28: | ||
Expression of the CMYC-6HIS-ADR and ADR-CMYC-6HIS recombinant proteins | Expression of the CMYC-6HIS-ADR and ADR-CMYC-6HIS recombinant proteins | ||
| − | + | https://2019.igem.org/wiki/images/6/67/T--Poitiers--recombinantexpression_ADR-tab3.png | |
Revision as of 09:17, 10 October 2019
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3038000
PCR amplification of the PCR products
Electrophoresis photography following deposits on agarose gel 0.8% of enzymatic digestion products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is the NEB 1 kb Plus Ladder. Lane 1 corresponds to the marker, lane 2 to the digested N-ter,ADR lane 3 to the digested C-ter ADR and lane 4 to the digested plasmid pSB1A3.
Plasmid construction
Design of ADR N-ter/pSB1A3 and ADR C-ter/pSB1A3 with Geneious software. This map shows the pBAD promoter and its terminator flanking the coding sequence of the ADR protein. Also present in N-ter or C-ter are 6-His and c-myc tag. Finally, in the plasmid is present and ampicillin resistance cassette.
Expression of the CMYC-6HIS-ADR and ADR-CMYC-6HIS recombinant proteins
Activity
User Reviews
UNIQ5bb6dca6fd5f2c1f-partinfo-00000000-QINU UNIQ5bb6dca6fd5f2c1f-partinfo-00000001-QINU