Difference between revisions of "Part:BBa K3038000:Experience"

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[[Image:T--Poitiers--PCR amplification ADR-tab3.jpg|400px|thumb|center| Electrophoresis photography following deposits on agarose gel 0.8% of enzymatic digestion products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is the NEB 1 kb Plus Ladder. Lane 1 corresponds to the marker, lane 2 to the digested N-ter,ADR lane 3 to the digested C-ter ADR and lane 4 to the digested plasmid pSB1A3.
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[[T--Poitiers--PCR amplification ADR-tab3.jpg|400px|thumb|center| Electrophoresis photography following deposits on agarose gel 0.8% of enzymatic digestion products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is the NEB 1 kb Plus Ladder. Lane 1 corresponds to the marker, lane 2 to the digested N-ter,ADR lane 3 to the digested C-ter ADR and lane 4 to the digested plasmid pSB1A3.
 
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Revision as of 08:39, 10 October 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3038000

PCR amplification of the PCR products


400px|thumb|center| Electrophoresis photography following deposits on agarose gel 0.8% of enzymatic digestion products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is the NEB 1 kb Plus Ladder. Lane 1 corresponds to the marker, lane 2 to the digested N-ter,ADR lane 3 to the digested C-ter ADR and lane 4 to the digested plasmid pSB1A3.


Plasmid construction



Expression of the recombinant protein


Activity

User Reviews

UNIQ856d6ac28ccc732e-partinfo-00000000-QINU UNIQ856d6ac28ccc732e-partinfo-00000001-QINU