Difference between revisions of "Part:BBa K3254006:Design"
Zongyeqing (Talk | contribs) (→References) |
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===Design Notes=== | ===Design Notes=== | ||
The sequence was first reported in 2014. | The sequence was first reported in 2014. | ||
| + | Two PstI site were eleminated by synonymous mutations. | ||
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===Source=== | ===Source=== | ||
| − | The original organism is the Streptomyces phage PhiK38-1.The DNA was | + | The original organism is the Streptomyces phage PhiK38-1. |
| + | The DNA was coloned from the source plasmid(pCis2+7+8+5, see reference). | ||
===References=== | ===References=== | ||
Yang, L., et al., Permanent genetic memory with >1-byte capacity. Nat Methods, 2014. 11(12): p. 1261-6. | Yang, L., et al., Permanent genetic memory with >1-byte capacity. Nat Methods, 2014. 11(12): p. 1261-6. | ||
Latest revision as of 13:13, 20 October 2019
Integrase Int5
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 30
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 22
Illegal AgeI site found at 406
Illegal AgeI site found at 781 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was first reported in 2014. Two PstI site were eleminated by synonymous mutations.
Source
The original organism is the Streptomyces phage PhiK38-1. The DNA was coloned from the source plasmid(pCis2+7+8+5, see reference).
References
Yang, L., et al., Permanent genetic memory with >1-byte capacity. Nat Methods, 2014. 11(12): p. 1261-6.