Difference between revisions of "Part:BBa K2978000:Design"

 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Synthesize <i>de novo</i> with codon optimization for <i>E.coli</i>
+
Synthesized <i>de novo</i> with codon optimization for <i>E.coli</i>. The sequence was obtained from Wang <i>et al.</i> work (2015).
  
 
===References===
 
===References===
 +
Wang, Q., Euler, C. W., Delaune, A. & Fischetti, V. A. (2015). Using a Novel Lysin To Help Control <i>Clostridium difficile</i> Infections. <i>Antimicrobial Agents and Chemotherapy</i>, 59(12), 7447–7457. doi:10.1128/aac.01357-15

Revision as of 18:10, 10 October 2019


Causes cell lysis of the pathogen Clostridium difficile


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Need to be express in a chassis that contains the T7 polymerase


Source

Synthesized de novo with codon optimization for E.coli. The sequence was obtained from Wang et al. work (2015).

References

Wang, Q., Euler, C. W., Delaune, A. & Fischetti, V. A. (2015). Using a Novel Lysin To Help Control Clostridium difficile Infections. Antimicrobial Agents and Chemotherapy, 59(12), 7447–7457. doi:10.1128/aac.01357-15