Difference between revisions of "Part:BBa K2938014"

Revision as of 09:16, 10 October 2019

Cry4Ba (HA tag) + deGFP Device

PR1 - RBS - Cry4Ba (HA tag) - RBS - deGFP - T500 Terminator

This plasmid was planned on a pBEST plasmid backbone. It contains larvicidal activity, as it containing toxic subunit of the bacillus thuringiensis israelensis (BTI) pBtoxis plasmid which is toxic to mosquitoes larva.

The plasmid was constructed using gibson assembly, then was incorporated into E.coli BL21 and DH5a using Heat shock, and into serratia marcescens 274 using electroporation.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1859
Illegal XbaI site found at 3597
Illegal SpeI site found at 2548
Illegal PstI site found at 363
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 56
Illegal NheI site found at 105
Illegal NheI site found at 247
Illegal SpeI site found at 2548
Illegal PstI site found at 363
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 4330
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1859
Illegal XbaI site found at 3597
Illegal SpeI site found at 2548
Illegal PstI site found at 363
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1859
Illegal XbaI site found at 3597
Illegal SpeI site found at 2548
Illegal PstI site found at 363
Illegal NgoMIV site found at 2671
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 653

@@ Line 4: / Line 4: @@
 PR1 - RBS - Cry4Ba (HA tag) - RBS - deGFP - T500 Terminator
+This plasmid was planned on a pBEST plasmid backbone.
+It contains larvicidal activity, as it containing toxic subunit of the bacillus thuringiensis israelensis (BTI) pBtoxis plasmid which is toxic to mosquitoes larva.
+The plasmid was constructed using gibson assembly, then was incorporated into E.coli BL21 and DH5a using Heat shock, and into serratia marcescens 274 using electroporation.
 <!-- Add more about the biology of this part here