Difference between revisions of "Part:BBa K2938016"

 
Line 3: Line 3:
 
<partinfo>BBa_K2938016 short</partinfo>
 
<partinfo>BBa_K2938016 short</partinfo>
  
−
PR1 - RBS - Cry11Aa (Strep tag) - RBS - P20 (His tag) - RBS - deGFP (His tag) - T500 Terminator
+
PR1 - RBS - Cry11Aa (Strep tag) - RBS - P20 (His tag) - RBS - deGFP - T500 Terminator
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:27, 9 October 2019


Cry11Aa (Strep tag) + P20 (His tag) + deGFP Device

PR1 - RBS - Cry11Aa (Strep tag) - RBS - P20 (His tag) - RBS - deGFP - T500 Terminator

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1870
    Illegal EcoRI site found at 2426
    Illegal XbaI site found at 2116
    Illegal XbaI site found at 2761
    Illegal SpeI site found at 982
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1870
    Illegal EcoRI site found at 2426
    Illegal NheI site found at 56
    Illegal NheI site found at 105
    Illegal SpeI site found at 982
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1870
    Illegal EcoRI site found at 2426
    Illegal XhoI site found at 3494
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1870
    Illegal EcoRI site found at 2426
    Illegal XbaI site found at 2116
    Illegal XbaI site found at 2761
    Illegal SpeI site found at 982
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1870
    Illegal EcoRI site found at 2426
    Illegal XbaI site found at 2116
    Illegal XbaI site found at 2761
    Illegal SpeI site found at 982
  • 1000
    COMPATIBLE WITH RFC[1000]