Difference between revisions of "Part:BBa K2936003"

Revision as of 20:41, 20 October 2019

S- glutathione hydrolase(Regulated by flhA gene)

This is the coding sequence of the S- glutathione hydrolase. The S- glutathione hydrolase oxidizes GSCHO to formic acid.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 130
Illegal PstI site found at 610
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 130
Illegal PstI site found at 610
Illegal NotI site found at 971
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 130
Illegal BglII site found at 491
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 130
Illegal PstI site found at 610
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 130
Illegal PstI site found at 610
Illegal NgoMIV site found at 79
Illegal NgoMIV site found at 275
Illegal NgoMIV site found at 628
Illegal NgoMIV site found at 888
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 This is the coding sequence of the S- glutathione hydrolase. The S- glutathione hydrolase oxidizes GSCHO to formic acid.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+<!-- Add more about the biology of this part here
+===Usage and Biology===
+Since the accumulation of formic acid would affect the growth of the strain, in order to further optimize the degradation efficiency of FDS-2 for formaldehyde, we found that LBFDH existing in L. buchneri could degrade formate into CO2. By introducing the gene controlling LBFDH expression, hoping to reduce the accumulation of formic acid in bacteria and improve the degradation efficiency of formate. ( Degradation principle is shown in Figure 1, and the plasmid map is shown in Figure2)
+<html>
+<img src="https://static.igem.org/mediawiki/parts/b/b4/T--ZJUT-China--igem-z4i1.png" width:700px>
+</html>
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>