Difference between revisions of "Part:BBa K3114008"

 
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Chlorophyll B Reductase is a naturally occurring plant enzyme, encoded by the gene NYC1, which catalyzes the first step of chlorophyll b degradation (Horie et al. 2009). Specifically, chlorophyll b reductase converts chlorophyll b into 7-Hydroxymethyl Chlorophyll a.
  
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===Usage and Biology===
 
===Usage and Biology===
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BBa_K3114008 is a golden gate compatible part which allows insertion of the NYC1 gene into genetic circuits to express the enzyme chlorophyll B reductase.
  
 
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===References===
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Horie, Y., Ito, H., Kusaba, M., Tanaka, R., & Tanaka, A. (2009). Participation of chlorophyll b reductase in the initial step of the degradation of light-harvesting chlorophyll a/b-protein complexes in Arabidopsis. Journal of Biological Chemistry, 284(26), 17449-17456.
 
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===Functional Parameters===
 
===Functional Parameters===
 
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Revision as of 04:03, 17 October 2019


Chlorophyll B Reductase (CBR) Chlorophyll B Reductase is a naturally occurring plant enzyme, encoded by the gene NYC1, which catalyzes the first step of chlorophyll b degradation (Horie et al. 2009). Specifically, chlorophyll b reductase converts chlorophyll b into 7-Hydroxymethyl Chlorophyll a.


Usage and Biology

BBa_K3114008 is a golden gate compatible part which allows insertion of the NYC1 gene into genetic circuits to express the enzyme chlorophyll B reductase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1061


References

Horie, Y., Ito, H., Kusaba, M., Tanaka, R., & Tanaka, A. (2009). Participation of chlorophyll b reductase in the initial step of the degradation of light-harvesting chlorophyll a/b-protein complexes in Arabidopsis. Journal of Biological Chemistry, 284(26), 17449-17456.