Difference between revisions of "Part:BBa K2908444:Design"

 
 
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===Design Notes===
 
===Design Notes===
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To be continued.
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- We designed, constructed, and sequence validated a miRNA sensor library containing all 620 sequences of mature human miRNAs designated as high confidence in miRBase 2124. Our library enables high-information-content screening of miRNA activity in cells and also serves as a source for sequence-validated templates of miRNA targets when building multi-input sensors. We synthesized miRNA target site sets bearing four repeats of the sequence perfectly complementary to the miRNA and inserted them into the 3′ UTR of a reporter construct.<br/>
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- It’s worth mentioning that, the design of this part is instructed by the 2019 team SYSU_CHINA (Detailed information of collaboration can be seen in the Team/Collaboration page in our wiki. Address: http://2019.igem.org/Team:CSU_CHINA/Collaborations)
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===References===
 
===References===
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Jeremy J. Gam, Jonathan Babb & Ron Weiss, NATURE COMMUNICATIONS (2018), A mixed antagonistic/synergistic miRNA repression model enables accurate predictions of multi-input miRNA sensor activity.

Latest revision as of 20:50, 17 October 2019


miR148b-BD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

- We designed, constructed, and sequence validated a miRNA sensor library containing all 620 sequences of mature human miRNAs designated as high confidence in miRBase 2124. Our library enables high-information-content screening of miRNA activity in cells and also serves as a source for sequence-validated templates of miRNA targets when building multi-input sensors. We synthesized miRNA target site sets bearing four repeats of the sequence perfectly complementary to the miRNA and inserted them into the 3′ UTR of a reporter construct.
- It’s worth mentioning that, the design of this part is instructed by the 2019 team SYSU_CHINA (Detailed information of collaboration can be seen in the Team/Collaboration page in our wiki. Address: http://2019.igem.org/Team:CSU_CHINA/Collaborations)



Source

This part is designed by assembling several complemental sequence with miR148b together and synthesized by company.

References

Jeremy J. Gam, Jonathan Babb & Ron Weiss, NATURE COMMUNICATIONS (2018), A mixed antagonistic/synergistic miRNA repression model enables accurate predictions of multi-input miRNA sensor activity.