Difference between revisions of "Part:BBa K3088003"
Line 6: | Line 6: | ||
− | |||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | |||
+ | DiphTox (DT) is a safe model of diphtheria toxin for research purpose to study its interaction with HBEGF receptor or HBEGF/ EnvZ chimeric receptor (BBa_K3088002) | ||
<!-- --> | <!-- --> |
Latest revision as of 11:33, 8 October 2019
DiphTox + RFP
This design is an improvement of the previous DiphTox (DT) (BBa_K2607000). We changed the Ribosomal Binding Site and added Red Fluorescent Protein (RFP) sequence following the DT protein. The addition of RFP may ease and improve post-transformation bacterial selection process in addition to antibiotic selection to prevent selecting bacteria that contains re-ligated plasmid.
Usage and Biology
DiphTox (DT) is a safe model of diphtheria toxin for research purpose to study its interaction with HBEGF receptor or HBEGF/ EnvZ chimeric receptor (BBa_K3088002)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 791
Illegal AgeI site found at 903 - 1000COMPATIBLE WITH RFC[1000]