Difference between revisions of "Part:BBa K3013000"
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LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene and polypropylene. It can be used to functionalize or tag polymers with proteins fused to the c-terminus of LCI. | LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene and polypropylene. It can be used to functionalize or tag polymers with proteins fused to the c-terminus of LCI. | ||
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| + | We used this part to tag polypropylene and polystyrene with eGFP. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | Therefore we made a fusion protein: eGFP - 17x helix - LCI. | ||
| + | This Construct was expressed under a T7 promoter in E. coli BL21. The soluble protein fraction was purified and binding was tested in microtiter plates. | ||
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Revision as of 10:03, 8 October 2019
LCI, polymer binding peptide
LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene and polypropylene. It can be used to functionalize or tag polymers with proteins fused to the c-terminus of LCI.
We used this part to tag polypropylene and polystyrene with eGFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]