Difference between revisions of "Part:BBa K3102025"

Revision as of 08:22, 12 October 2019

T7-RBS-TEVp-ter

This T7-TEVp-TEV-hlyA-ter (secreted TEV) composite is engaged into the TEV protease synthetization and secretion.

The TEV (BBa_K3102012) and HlyA basic parts (BBa_K3102015 or BBa_K554002) are present in this composite. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV peptide signal is used as a recognition signal to remove the signal peptide, HlyA by the TEV protease.

This constructs is enriched with primer, T7 promoters, RBS and terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 541
Illegal NgoMIV site found at 847
Illegal AgeI site found at 202
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 This T7-TEVp-TEV-hlyA-ter (secreted TEV) composite is engaged into the TEV protease synthetization and secretion.
-The TEV (BBa_K3102012) and HlyA basic parts (BBa_K3102015 or BBa_K554002) are present in this composite. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV part added a TEV recognition sequence, that will be used to remove the signal peptide, HlyA.
+The TEV (BBa_K3102012) and HlyA basic parts (BBa_K3102015 or BBa_K554002) are present in this composite. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV peptide signal is used as a recognition signal to remove the signal peptide, HlyA by the TEV protease.
 This constructs is enriched with primer, T7 promoters, RBS and terminator.