Difference between revisions of "Part:BBa K3102024"
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<partinfo>BBa_K3102024 short</partinfo> | <partinfo>BBa_K3102024 short</partinfo> | ||
| − | This T7-EG1-TEV-hlyA-ter (secreted EG1) composite is engaged into the cellulose acetate breaking down, by synthesize the EG1 (Endo-1,4-beta-glucanase). The TEV ( | + | This T7-EG1-TEV-hlyA-ter (secreted EG1) composite is engaged into the cellulose acetate breaking down, by synthesize the EG1 (Endo-1,4-beta-glucanase). The TEV (BBa_K3102041) and HlyA basic parts (BBa_K554002) are present in this composite. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV peptide signal is used as a recognition signal to remove the signal peptide, HlyA by the TEV protease. |
| − | + | ||
This constructs is enriched with primer, T7 promoters, RBS and terminator. | This constructs is enriched with primer, T7 promoters, RBS and terminator. | ||
Revision as of 08:14, 12 October 2019
T7-RBS-EG1-ter (secreted EG1)
This T7-EG1-TEV-hlyA-ter (secreted EG1) composite is engaged into the cellulose acetate breaking down, by synthesize the EG1 (Endo-1,4-beta-glucanase). The TEV (BBa_K3102041) and HlyA basic parts (BBa_K554002) are present in this composite. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV peptide signal is used as a recognition signal to remove the signal peptide, HlyA by the TEV protease. This constructs is enriched with primer, T7 promoters, RBS and terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1161
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 104
Illegal AgeI site found at 362
Illegal AgeI site found at 452
Illegal AgeI site found at 1289 - 1000COMPATIBLE WITH RFC[1000]