Difference between revisions of "Part:BBa K3102021"

Revision as of 17:07, 12 October 2019

ACS (Acetyl-coenzyme A synthetase) Escherichia.coli

ACS (Acetyl-coenzyme A synthetase) activity constitutes one of two distinct pathways by which E. coli activates acetate to acetyl-CoA in an ATP-dependent manner. [Brown et al., 1997; Kumari et al., 1995] The ACS pathway functions in a mainly anabolic role, scavenging acetate present in the extracellular medium. The ACS gene encoding this enzyme is overexpressed to enhance acetyl-CoA's generation.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 373
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 802
1000
COMPATIBLE WITH RFC[1000]

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 ACS (Acetyl-coenzyme A synthetase) activity constitutes one of two distinct pathways by which E. coli activates acetate to acetyl-CoA in an ATP-dependent manner. [Brown et al., 1997; Kumari et al., 1995] The ACS pathway functions in a mainly anabolic role, scavenging acetate present in the extracellular medium. The ACS gene encoding this enzyme is overexpressed to enhance acetyl-CoA's generation.
-Brown TD. et al., The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli, J Gen Microbiol. 1977 Oct;102(2):327-36.
-Kumari S. et al., Cloning, characterization, and functional expression of acs, the gene which encodes acetyl coenzyme A synthetase in Escherichia coli, J Bacteriol. 1995 May;177(10):2878-86.
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