Difference between revisions of "Part:BBa K3102033"

 
 
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<partinfo>BBa_K3102033 short</partinfo>
 
<partinfo>BBa_K3102033 short</partinfo>
  
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This T7-RBS-GND-ter composite part is made of the 6-phosphogluconate dehydrogenase enzyme  from Corynebacterium glutamicum catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH in the pentose phosphate pathway. This enzyme catalyzes the following reactions after ZWF and PGL genes. This part is useful to increase flavin production.
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This construct is enriched with T7 promoter, RBS and terminator.
  
 
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Latest revision as of 13:22, 14 October 2019


T7-RBS-GND-Ter

This T7-RBS-GND-ter composite part is made of the 6-phosphogluconate dehydrogenase enzyme from Corynebacterium glutamicum catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH in the pentose phosphate pathway. This enzyme catalyzes the following reactions after ZWF and PGL genes. This part is useful to increase flavin production.

This construct is enriched with T7 promoter, RBS and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 503
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 233