Difference between revisions of "Part:BBa I746916:Experience"

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[[Image:T--Linkoping_Sweden--CBD-sfGFPstorodl4.jpeg|300px|thumb|left|<b><i>Figure 1.</i></b> 1 liter growth medium containing induced CBD-sfGFP BL21 (DE3) on a UV-table. ]]  
 
[[Image:T--Linkoping_Sweden--CBD-sfGFPstorodl4.jpeg|300px|thumb|left|<b><i>Figure 1.</i></b> 1 liter growth medium containing induced CBD-sfGFP BL21 (DE3) on a UV-table. ]]  
  
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<b>Figure 1</b> present a 1 liter culture of induced CBD-sfGFP E.coli BL21(DE3) on a UV-table. The picture shows that an induced culture of CBD-sfGFP emits fluorescence at 302 nm.
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<b>Figure 1</b> present a 1 liter culture of induced E.coli BL21(DE3) CBD-sfGFP on a UV-table. The picture shows that an induced culture of CBD-sfGFP fluoresces at 302 nm.
  
  

Revision as of 09:40, 6 October 2019


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Applications of BBa_I746916

2019 iGEM team Linkoping Sweden

Figure 1. 1 liter growth medium containing induced CBD-sfGFP BL21 (DE3) on a UV-table.

Figure 1 present a 1 liter culture of induced E.coli BL21(DE3) CBD-sfGFP on a UV-table. The picture shows that an induced culture of CBD-sfGFP fluoresces at 302 nm.




















Pictures above shows BL21 DE3 and Vibrio natriegens Vmax expressing CBD-sfGFP at different IPTG concentrations between 0.05 - 1.0 mM. The induction gradient for Vibrio is more correlated with the IPTG concentration then E.coli suggesting that the induction windows for expressing CBD-sfGFP are different between E.coli BL21 (DE3) and Vibrio natriegens Vmax.




















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