Difference between revisions of "Part:BBa K3034004"
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===Results=== | ===Results=== | ||
| − | We showed the growth of E. coli by measuring the growth curve of the Abs600 value of the bacterial liquid every hour. The results are as follows: | + | We showed the growth of ''E. coli'' by measuring the growth curve of the Abs600 value of the bacterial liquid every hour. The results are as follows: |
| + | [[File:BBa_K3034004-growth_curve_of_BL21(DE3).png|700px|thumb|center|'''Fig.1''' Characterization of intracellular cleavage effect of Lysep3-D8 protein(37˚C). The experimental data comes from two sets of biological replicates, repeated three times in each group.]] | ||
| + | Figure 1 shows that when the expression of Lysep3-D8 was induced by the addition of IPTG at a final concentration of 0.5 mM, the difference in Abs600 between the experimental group (+IPTG) and the control group (-IPTG) was larger and larger. That is, the Lysep3-D8 protein inhibited the growth of Escherichia coli (BL21) (inhibition rate was about 30.7%). | ||
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Revision as of 08:47, 6 October 2019
The fusion protein is an enhanced lysin-> lysep3-D8
This lysin is formed by fusion of lysep3 and D8,which lyses bacteria from inside and outside the cell.The bactericidal spectrum of lysep3-D8 is broad, as it can lyse both gram-negative bacteria and gram-positive bacteria(14 E. coli strains, 3 P. aeruginosa strains, 1 Acinetobacter baumannii strain,and 1 Streptococcus strain).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 79
- 1000COMPATIBLE WITH RFC[1000]