Difference between revisions of "Part:BBa K3100011"
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<partinfo>BBa_K3100011 short</partinfo> | <partinfo>BBa_K3100011 short</partinfo> | ||
| + | <h2><strong>Usage and Biology:</strong></h2> | ||
| + | The toehold switches are classes of riboregulators that are de-novo-designed based on influence of secondary structure on mRNA translation and knowledge of RNA thermodynamics and kinetics. Toehold switch systems are composed of two RNA strands referred to as the switch and trigger. The switch RNA contains the coding sequence of the gene being regulated. Upstream of this coding sequence is a hairpin-based processing module containing both a strong RBS and a start codon. The trigger RNA can specifically bind to a single-stranded toehold sequence at the 5’ end of the hairpin module, and expose the RBS and start codon by a branch migration process that depends on the complement of an extended single-stranded region and the hairpin, thereby initiating translation.<br> | ||
| + | <center>[[File:T--SCUT China--toe tri.jpeg|500px]]</center><br> | ||
| + | <center>Fig, 1: Toehold Switch and Trigger RNA<center><br> | ||
| + | The new classes of regulatory components offer wide dynamic range and low system crosstalk. We selected four toehold switches with widest dynamic range and highest orthogonality used for multiplexing systems and provided by the reference, which are switch A&B&C&D and trigger A&B&C&D, to regulate the expressions of four acid-resistance factors. <br> | ||
| − | + | <h2><strong>Characterization:</strong></h2> | |
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Revision as of 11:45, 21 October 2019
toehold switch A
Usage and Biology:
The toehold switches are classes of riboregulators that are de-novo-designed based on influence of secondary structure on mRNA translation and knowledge of RNA thermodynamics and kinetics. Toehold switch systems are composed of two RNA strands referred to as the switch and trigger. The switch RNA contains the coding sequence of the gene being regulated. Upstream of this coding sequence is a hairpin-based processing module containing both a strong RBS and a start codon. The trigger RNA can specifically bind to a single-stranded toehold sequence at the 5’ end of the hairpin module, and expose the RBS and start codon by a branch migration process that depends on the complement of an extended single-stranded region and the hairpin, thereby initiating translation.
The new classes of regulatory components offer wide dynamic range and low system crosstalk. We selected four toehold switches with widest dynamic range and highest orthogonality used for multiplexing systems and provided by the reference, which are switch A&B&C&D and trigger A&B&C&D, to regulate the expressions of four acid-resistance factors.
Characterization:
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]