Difference between revisions of "Plasmid backbones/Measurement"
| Line 9: | Line 9: | ||
*There is a kit of components available that is described [[Measurement|here]] to measure promoter activity in standard units. The kit makes use of [[Part:pSB3K3]] as the plasmid and [[Part:BBa_E0240]] (GFP) to measure promoter activity. | *There is a kit of components available that is described [[Measurement|here]] to measure promoter activity in standard units. The kit makes use of [[Part:pSB3K3]] as the plasmid and [[Part:BBa_E0240]] (GFP) to measure promoter activity. | ||
| − | ===Inverters | + | ===Inverters=== |
| − | *[[Part:pSB1A10]] - This dual reporter (GFP and RFP) plasmid can be used to measure the transfer curve of [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=inverter | + | *[[Part:pSB1A10]] - This dual reporter (GFP and RFP) plasmid can be used to measure the transfer curve of [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=inverter inverter devices]. |
Revision as of 13:23, 5 September 2008
| Part assembly | System operation | Protein expression | Assembly of protein fusions | Part measurement | Screening of part libraries | Building BioBrick vectors | DNA synthesis | Other standards | Archive |
| Or get some help on plasmid backbones. |
Some plasmids are designed to facilitate the measurement of different part classes:
Promoters
- Part:BBa_J23101 - A collection of promoters characterized by Chris Anderson. The plasmid backbone from these parts can be used to measure the activity of other promoters based on expression of RFP.
- There is a kit of components available that is described here to measure promoter activity in standard units. The kit makes use of Part:pSB3K3 as the plasmid and Part:BBa_E0240 (GFP) to measure promoter activity.
Inverters
- Part:pSB1A10 - This dual reporter (GFP and RFP) plasmid can be used to measure the transfer curve of inverter devices.