Difference between revisions of "Part:BBa K3076200:Design"
 
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===Design Notes===
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===Source===
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This part contains the coding sequence of vanabin2 gene. It was identified from literature review [1] and extracted from NCBI (ACCESSION: AB088204) starting from start codon to stop codon. Then the sequence was codon optimized for E. coli by ThermoFisher GeneArt platform.  
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This part contains the coding sequence of the <i>vanabin2</i> gene. It was identified from the literature review [1] and extracted from NCBI (ACCESSION: AB088204) starting from start codon to stop codon. Then the sequence was codon-optimized for <i>E. coli</i> by the ThermoFisher GeneArt platform.  
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===Design Consideration===
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===Design notes===
 
Overlapping sequence of pET28a multiple cloning site was added to flank the CDS for cloning by Hifi assembly.
 
Overlapping sequence of pET28a multiple cloning site was added to flank the CDS for cloning by Hifi assembly.
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===Reference===
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===References===
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[1] Ueki, T., Sakamoto, Y., Yamaguchi, N., & Michibata, H. (2003). Bioaccumulation of Copper Ions by Escherichia coli Expressing Vanabin Genes from the Vanadium-Rich Ascidian <i>Ascidia sydneiensis samea</i>. Applied and Environmental Microbiology, 69(11), 6442–6446. doi: 10.1128/aem.69.11.6442-6446.2003
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[1] Ueki, T., Sakamoto, Y., Yamaguchi, N., & Michibata, H. (2003). Bioaccumulation of Copper Ions by Escherichia coli Expressing Vanabin Genes from the Vanadium-Rich Ascidian Ascidia sydneiensis samea. Applied and Environmental Microbiology, 69(11), 6442–6446. doi: 10.1128/aem.69.11.6442-6446.2003
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Latest revision as of 12:48, 11 October 2019


Coding sequence of Vanabin2 gene from Ascidia sydneiensis samea


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 90
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

This part contains the coding sequence of the vanabin2 gene. It was identified from the literature review [1] and extracted from NCBI (ACCESSION: AB088204) starting from start codon to stop codon. Then the sequence was codon-optimized for E. coli by the ThermoFisher GeneArt platform.

Design notes

Overlapping sequence of pET28a multiple cloning site was added to flank the CDS for cloning by Hifi assembly.

Reference

[1] Ueki, T., Sakamoto, Y., Yamaguchi, N., & Michibata, H. (2003). Bioaccumulation of Copper Ions by Escherichia coli Expressing Vanabin Genes from the Vanadium-Rich Ascidian Ascidia sydneiensis samea. Applied and Environmental Microbiology, 69(11), 6442–6446. doi: 10.1128/aem.69.11.6442-6446.2003