Difference between revisions of "Part:BBa K2967013"

Revision as of 06:08, 5 October 2019

Amplifier system with hIL-10.

Amplifier system is created by our team NEU-CHINA 2019. To make sure that amplifier is able to strengthen the expression of gene downstream to promoter hrpL. A coomassie brilliant blue is demonstrated to show that more protein at 35kDA, which should be IL-10, is witnessed when introducing IL-10 gene downstream of promoter hrpL. And it can be seen that lanes with amplifier seems to be darker, which means that this amplifier system tends to strengthen overall protein expression.

Figure 1. Expressing vector is transformed into BL21 strain. CFU is then inoculated to LB medium followed by 12h incubation at 37℃. After induction of IPTG, final concentration at 1mM, the culture is incubated at 37℃ for 2h. A coomassie brilliant blue is demonstrated to show the result. The red box shows where the target protein is.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2082
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1292
Illegal SapI.rc site found at 1925

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2967013 short</partinfo>
-Amplifier system is created by our team NEU-CHINA 2019. To make sure that amplifier is able to strengthen the expression of gene downstream to promoter hrpL. A coomassie brilliant blue is demonstrated to show that more protein at 35kDA, which should be IL-10, is witnessed when introducing IL-10 gene downstream of promoter hrpL.
+Amplifier system is created by our team NEU-CHINA 2019. To make sure that amplifier is able to strengthen the expression of gene downstream to promoter hrpL. A coomassie brilliant blue is demonstrated to show that more protein at 35kDA, which should be IL-10, is witnessed when introducing IL-10 gene downstream of promoter hrpL. And it can be seen that lanes with amplifier  seems to be darker, which means that this amplifier system tends to strengthen overall protein expression.
 https://static.igem.org/mediawiki/parts/thumb/1/1b/T--NEU_China--part--amp%2Bil-10-1.png/800px-T--NEU_China--part--amp%2Bil-10-1.png
-Figure 1. Expressing vector is transformed into BL21 strain. CFU is then inoculated to LB medium followed by 12h incubation at 37℃. After induction of IPTG, final concentration at 1mM, the culture is incubated at 37℃ for 2h. A coomassie brilliant blue is demonstrated to show the result.
+Figure 1. Expressing vector is transformed into BL21 strain. CFU is then inoculated to LB medium followed by 12h incubation at 37℃. After induction of IPTG, final concentration at 1mM, the culture is incubated at 37℃ for 2h. A coomassie brilliant blue is demonstrated to show the result. The red box shows where the target protein is.
 <!-- Add more about the biology of this part here