Difference between revisions of "Part:BBa K3185005"
Line 2: | Line 2: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K3185005 short</partinfo> | <partinfo>BBa_K3185005 short</partinfo> | ||
+ | ==Usage and Biology== | ||
− | + | <br> | |
− | + | <br> | |
− | < | + | |
− | + | ||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K3185004 SequenceAndFeatures</partinfo> | ||
+ | ==Purification== | ||
+ | <br> | ||
+ | <h3><font size="4.5">Expression</font> </h3> | ||
+ | <ul> | ||
+ | <li>Cells were grown in 200ml LB media (100μg/ml Ampicillin) at 37<sup>o</sup>C shaking at 140 rpm to an OD600 of 0.5, verifying via a spectrophotometer. | ||
+ | </ul> | ||
+ | <ul> | ||
+ | <li>Protein was expressed in 0.1mM IPTG for 2hours. | ||
+ | </ul> | ||
+ | <h3><font size="4.5">SDS-PAGE</font></h3> | ||
+ | <br> | ||
+ | <br> | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 13:34, 17 October 2019
SPYCatcher -> CenA W68Y
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Purification
Expression
- Cells were grown in 200ml LB media (100μg/ml Ampicillin) at 37oC shaking at 140 rpm to an OD600 of 0.5, verifying via a spectrophotometer.
- Protein was expressed in 0.1mM IPTG for 2hours.
SDS-PAGE
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 751
Illegal AgeI site found at 841 - 1000COMPATIBLE WITH RFC[1000]