Difference between revisions of "Part:BBa K2996008"
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The expresssion of cas1-cas2 complex can be triggered by the addition of AHL.
 
The expresssion of cas1-cas2 complex can be triggered by the addition of AHL.
  
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#Usage and Biology
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#usage
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Expression of the CRISPR adaptation proteins Cas1 and Cas2 promotes unidirectional integration of 33–base pair DNA spacers into our designed RSRL array.
 
Expression of the CRISPR adaptation proteins Cas1 and Cas2 promotes unidirectional integration of 33–base pair DNA spacers into our designed RSRL array.
  
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On the other hand, the input information is now acyl-homoserine lactones. In the transcrption activation gene circuit, luxI can be put downstream of promotor J23001, enabling the expression of luxl enzyme when dcas9 binds to lure sequence. AHL molecule production will then activate the expresssion of cas1-cas2 complex, in this way, the off-targte incidence can be recorded.
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On the other hand, the input information is now acyl-homoserine lactones. In the transcription activation gene circuit, luxI can be put downstream of promotor J23001, enabling the expression of Luxl enzyme when dcas9 binds to lure sequence. AHL molecule production will then activate the expression of cas1-cas2 complex, in this way, the off-target incidence can be recorded.
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#Result
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蛋白胶图
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Revision as of 01:10, 11 October 2019


pLuxR upstream of cas1/2

The expresssion of cas1-cas2 complex can be triggered by the addition of AHL.

  1. Usage and Biology

Expression of the CRISPR adaptation proteins Cas1 and Cas2 promotes unidirectional integration of 33–base pair DNA spacers into our designed RSRL array.

On the other hand, the input information is now acyl-homoserine lactones. In the transcription activation gene circuit, luxI can be put downstream of promotor J23001, enabling the expression of Luxl enzyme when dcas9 binds to lure sequence. AHL molecule production will then activate the expression of cas1-cas2 complex, in this way, the off-target incidence can be recorded.


  1. Result

蛋白胶图


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 258
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]