Difference between revisions of "Part:BBa K3221202:Design"

 
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===Source===
 
===Source===
  
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We get the pMET3 from the BY4741 genomic, and the plasmid with yeGFP from the Team Peking. The CYC1 terminator is initially on the pYES2.
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We get the pMET3 from the BY4742 genomic, and the plasmid with yeGFP from the Team Peking. The CYC1 terminator is initially on the pYES2.
  
 
===References===
 
===References===

Revision as of 09:33, 4 October 2019


pMET3-yeGFP-CYC1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 302
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 399
    Illegal BsaI.rc site found at 1144


Design Notes

We design this part to fit the RFC10.


Source

We get the pMET3 from the BY4742 genomic, and the plasmid with yeGFP from the Team Peking. The CYC1 terminator is initially on the pYES2.

References