Difference between revisions of "Part:BBa K3098021"

Revision as of 17:06, 16 October 2019

Omp Promoter + riboswitch/sfGFP

The promoter pOmpC is positively regulated, OmpR-controlled promoter (from Part: BBa_RB0082 ). This promoter is taken from the upstream region of OmpC. Phosphorylated OmpR binds to the three operator sites and activates transcription. Its expression is related to the osmotic pressure. In nature, this promoter is upstream of the OmpC porin gene. The regulation of OmpC is determined by the EnvZ-OmpR osmosensing machinery. EnvZ phosphorylates OmpR to OmpR-P. At high osmolarity, EnvZ is more active, creating more OmpR-P. OmpR-P then binds to the low-affinity OmpR operator sites upstream of ompC.

But we found that, this promoter can be used not only for its osmosensing function, in some other condition, it is also be used as a part of a system, such as the red light system ( from Part: BBa_K1017301). In such condition, we hope to get rid of the influence of the osmosis, thus, we create a new part by adding a riboswitch after the promoter, to realize another kind of control via theophylline.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 822

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3098021 short</partinfo>
-In order to rezlize the red light control of our target gene, we construct the whole gene circuit on the basis  of iGEM13_NCTU_Formosa's work. We integrated each parts of this light system, and change the NOT gate in order to achieve better effects.
+The promoter pOmpC is positively regulated, OmpR-controlled promoter (from Part: BBa_RB0082 ). This promoter is taken from the upstream region of OmpC. Phosphorylated OmpR binds to the three operator sites and activates transcription. Its expression is related to the osmotic pressure. In nature, this promoter is upstream of the OmpC porin gene. The regulation of OmpC is determined by the EnvZ-OmpR osmosensing machinery. EnvZ phosphorylates OmpR to OmpR-P. At high osmolarity, EnvZ is more active, creating more OmpR-P. OmpR-P then binds to the low-affinity OmpR operator sites upstream of ompC.
+But we found that, this promoter can be used not only for its osmosensing function, in some other condition, it is also be used as a part of a system, such as the red light system ( from Part: BBa_K1017301). In such condition, we hope to get rid of the influence of the osmosis, thus, we create a new part by adding a riboswitch after the promoter, to realize another kind of control via theophylline.
 <!-- Add more about the biology of this part here