Difference between revisions of "Part:BBa K3102015"
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| + | HLYA signal peptide found in the C-terminal signal sequence of alpha-hemolysin. Used to target proteins for secretion via the Type I secretion pathway of gram-negative bacteria [Nicaud J. M. et al., 1986; Stanley P. et al., 1991]. Unlike Type II signal peptides, fusion of the HlyA signal peptide to the target protein may result in transport of the protein from the cytoplasm to the extracellular medium in a single step. | ||
| + | For our project, this protein is used to secrete both CAE and EG1 proteins by E.coli. | ||
Revision as of 09:16, 14 October 2019
HLYA signal peptide found in the C-terminal signal sequence of alpha-hemolysin. Used to target proteins for secretion via the Type I secretion pathway of gram-negative bacteria [Nicaud J. M. et al., 1986; Stanley P. et al., 1991]. Unlike Type II signal peptides, fusion of the HlyA signal peptide to the target protein may result in transport of the protein from the cytoplasm to the extracellular medium in a single step.
For our project, this protein is used to secrete both CAE and EG1 proteins by E.coli.