Difference between revisions of "Part:BBa K3102013"

 
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This ACS operon is composed of three genes: actP (Transports acetate) acs (catalyzes the conversion of acetate into acetyl-CoA) and yfcH (epimerase)[Gimenez R et al., 2003]. This part is useful to increase the overexpression of the operon and thus, the uptake of acetate into the cell to metabolize it into other substrates.
 
This ACS operon is composed of three genes: actP (Transports acetate) acs (catalyzes the conversion of acetate into acetyl-CoA) and yfcH (epimerase)[Gimenez R et al., 2003]. This part is useful to increase the overexpression of the operon and thus, the uptake of acetate into the cell to metabolize it into other substrates.
  
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Gimenez R et al., The Gene yjcG, Cotranscribed with the Gene acs, Encodes an Acetate Permease in Escherichia coli, Journal of Bacteriology, 2003 Nov; 185(21):6448-55.
 
  
 
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Revision as of 17:01, 12 October 2019


ACS Operon (acs, yfcH, actP)

This ACS operon is composed of three genes: actP (Transports acetate) acs (catalyzes the conversion of acetate into acetyl-CoA) and yfcH (epimerase)[Gimenez R et al., 2003]. This part is useful to increase the overexpression of the operon and thus, the uptake of acetate into the cell to metabolize it into other substrates.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 373
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 802
    Illegal AgeI site found at 2393
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3667