Difference between revisions of "Part:BBa K2996501:Design"
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===Source=== | ===Source=== | ||
| − | + | The subparts of this composite part were BBa_K2996500 and BBa_B0010.BBa_B0010 was added through one additional PCR on the basis of BBa_K2996500. | |
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===References=== | ===References=== | ||
Revision as of 06:51, 3 October 2019
Terminator put downstream of zinc finger
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 304
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We wanted a relatively strong terminator that can stop the transcription of DBD protein,thus lowering the leakage in the system,so we chose the terminator BBa_B0010 as the terminator.We designed new PCR primers to extend the original DNA sequence of DBD protein(BBa_K2996500),adding the DNA sequence of BBa_B0010 to the sequence.
Source
The subparts of this composite part were BBa_K2996500 and BBa_B0010.BBa_B0010 was added through one additional PCR on the basis of BBa_K2996500.