Difference between revisions of "Part:BBa K2996500"
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<partinfo>BBa_K2996500 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2996500 SequenceAndFeatures</partinfo> | ||
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| + | <center><img style="max-width: 80%" src="https://2019.igem.org/wiki/images/e/e2/T--SJTU-BioX-Shanghai--jsf-NDBD.png" alt="" /></center> | ||
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| + | <center><b>Figure 1.</b> <i>Fig.2 PCR product of DBD with rrnB T1 terminator downstream.(Lane1:product of DBD;Lane 2:2000bp ladder).</i> </center> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2996500 parameters</partinfo> | <partinfo>BBa_K2996500 parameters</partinfo> | ||
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Revision as of 23:40, 21 October 2019
Zinc finger with flag tag
It is a sequence encoding protein that can recruit zinc ions for functioning.The protein can bind specific DNA sequence 5'-aTTAGGAGTGa-3'.To better purify the protein,we also added a flag tag at the C terminal of it. It is composed of mammalian synthetic transcription factors (SynTFs) based on Cys2-His2 zinc finger 21-16.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 304
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
