Difference between revisions of "Part:BBa K2967019"
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In order to see whether the nitric oxide sensor will work when it comes to expressing a certain protein, we add the IL-10 downstream and demonstrated the western blotting to show the result. It has been proved that the expression level can be induced when adding nitric oxide. | In order to see whether the nitric oxide sensor will work when it comes to expressing a certain protein, we add the IL-10 downstream and demonstrated the western blotting to show the result. It has been proved that the expression level can be induced when adding nitric oxide. | ||
| + | https://static.igem.org/mediawiki/parts/thumb/7/7e/T--NEU_China--part--pyear-il10-1.png/800px-T--NEU_China--part--pyear-il10-1.png | ||
| + | https://static.igem.org/mediawiki/parts/thumb/3/3c/T--NEU_China--part--pyear%2Bli10-2.png/800px-T--NEU_China--part--pyear%2Bli10-2.png | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 07:08, 3 October 2019
The yeaR promoter will initiate the express of IL-10 when nitric oxide appear
In order to see whether the nitric oxide sensor will work when it comes to expressing a certain protein, we add the IL-10 downstream and demonstrated the western blotting to show the result. It has been proved that the expression level can be induced when adding nitric oxide.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]