Difference between revisions of "Part:BBa K2970003"
Line 3: | Line 3: | ||
<partinfo>BBa_K2970003 short</partinfo> | <partinfo>BBa_K2970003 short</partinfo> | ||
− | Together with Trigger 2, it forms a construct that can open the gate. | + | Together with Trigger 2 (<partinfo>BBa_K2970001</partinfo>), it forms a construct that can open the gate (<partinfo>BBa_K2970002</partinfo>). |
The affinity between the trigger complex and the gate is greater than that of the gate to itself (loop). A single trigger cannot open the gate because it has only half the required complementary sequence. | The affinity between the trigger complex and the gate is greater than that of the gate to itself (loop). A single trigger cannot open the gate because it has only half the required complementary sequence. | ||
+ | |||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 16:59, 2 October 2019
Trigger 1 Composition
Together with Trigger 2 (BBa_K2970001), it forms a construct that can open the gate (BBa_K2970002). The affinity between the trigger complex and the gate is greater than that of the gate to itself (loop). A single trigger cannot open the gate because it has only half the required complementary sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]