Difference between revisions of "Part:BBa K3098025"

 
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<partinfo>BBa_K3098025 short</partinfo>
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Free radicals, such as superoxide anions (O2·-) or hydroxyl radicals (HO·), can cause protein damage and DNA mutations that can initiate several diseases, including coronary heart diseases and diabetes. Antioxidants that can scavenge free radicals play an important role in biological systems.An antioxidant peptide derived from Pinctada fucata has great poteintial for scavenging free radicals. Howeverthe the low rates of enzymatic hydrolysis extraction and purification, as well as the higher costs for purifying from Pinctada fucata meat, suggest that this natural and safe antioxidant peptide would not meet industrialized production requirements. Thus we have submited a designed AOP that can be easily produced and purified in E.coli, named 'DSAOP' BBa_K3098024.
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For our project need to assemble this DSAOP onto the Bacteria cellulose via a connection of streptomycin and biotin. Thus we add Avitag onto the N terminal of DSAOP which can link with biotin under the catalyzation of the enzyme BirA K2485001.So that DSAOP can be biotinated during its production in E.coli.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3098025 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3098025 parameters</partinfo>
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Revision as of 05:31, 2 October 2019


DSAOP+Avi-tag

Free radicals, such as superoxide anions (O2·-) or hydroxyl radicals (HO·), can cause protein damage and DNA mutations that can initiate several diseases, including coronary heart diseases and diabetes. Antioxidants that can scavenge free radicals play an important role in biological systems.An antioxidant peptide derived from Pinctada fucata has great poteintial for scavenging free radicals. Howeverthe the low rates of enzymatic hydrolysis extraction and purification, as well as the higher costs for purifying from Pinctada fucata meat, suggest that this natural and safe antioxidant peptide would not meet industrialized production requirements. Thus we have submited a designed AOP that can be easily produced and purified in E.coli, named 'DSAOP' BBa_K3098024. For our project need to assemble this DSAOP onto the Bacteria cellulose via a connection of streptomycin and biotin. Thus we add Avitag onto the N terminal of DSAOP which can link with biotin under the catalyzation of the enzyme BirA K2485001.So that DSAOP can be biotinated during its production in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 144
    Illegal BamHI site found at 187
    Illegal XhoI site found at 385
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]