Difference between revisions of "Part:BBa K3098014:Design"
| Line 1: | Line 1: | ||
| − | + | The sequence of antioxidant peptides isolated from Pinctada fucata meat typically includes 10 to 20 amino acids, which is relatively short and easily degraded by protease or peptidase in Escherichia coli expression systems. Therefore, it is difficult to express these peptides directly. | |
| + | Based on previous reports that an antioxidant peptide from Pinctada fucata meat (amino acid sequence was GAGLPGKRER, named AOP) had high antioxidant activity in vitro, a design containing the GAGLPGKRER sequence was constructed for further expression to obtain a higher yield and improve its stability in our project’s applications in room temperature environment. Meanwhile,and solubility of the designed polypeptide were considered. Finally, The DSAOP DNA sequence encoding 113 amino acids was synthesized and codon-optimized for E. coli expression using Primer Premier 5.0. | ||
Latest revision as of 04:18, 2 October 2019
The sequence of antioxidant peptides isolated from Pinctada fucata meat typically includes 10 to 20 amino acids, which is relatively short and easily degraded by protease or peptidase in Escherichia coli expression systems. Therefore, it is difficult to express these peptides directly. Based on previous reports that an antioxidant peptide from Pinctada fucata meat (amino acid sequence was GAGLPGKRER, named AOP) had high antioxidant activity in vitro, a design containing the GAGLPGKRER sequence was constructed for further expression to obtain a higher yield and improve its stability in our project’s applications in room temperature environment. Meanwhile,and solubility of the designed polypeptide were considered. Finally, The DSAOP DNA sequence encoding 113 amino acids was synthesized and codon-optimized for E. coli expression using Primer Premier 5.0.