Difference between revisions of "Part:BBa K2992000"

(Usage and Biology)
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===Characterisation===
 
===Characterisation===
Date incoming <br><br><br>
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This part was used as part of our FAST reporter constructs - [https://parts.igem.org/Part:BBa_K2992042 BBa_K2992042],[https://parts.igem.org/Part:BBa_K2992043 BBa_K2992043],[https://parts.igem.org/Part:BBa_K2992044 BBa_K2992044],[https://parts.igem.org/Part:BBa_K2992045 BBa_K2992045],
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[https://parts.igem.org/Part:BBa_K2992046 BBa_K2992046], [https://parts.igem.org/Part:BBa_K2992047 BBa_K2992047],[https://parts.igem.org/Part:BBa_K2992048 BBa_K2992048] - which were characterised using the FAST fluorometric assay....  <br><br><br>
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===

Revision as of 12:08, 21 October 2019


FAST reporter gene

Fluorescence-Activating and Absorption-Shifting Tag Protein (FAST) reporter gene.

Usage and Biology

FAST is one of the few fluorescent reporters available for effective use in anaerobic organisms. FAST is derived from Halorhodospira halophila and has been codon optimised for fluorescence studies in the genus Clostridium. In our project we use FAST as a reporter to demonstrate the activity of our chosen promoters and to assess its suitability as an alternative non-volatile reporter system for predicting neurotoxin production.


Characterisation

This part was used as part of our FAST reporter constructs - BBa_K2992042,BBa_K2992043,BBa_K2992044,BBa_K2992045, BBa_K2992046, BBa_K2992047,BBa_K2992048 - which were characterised using the FAST fluorometric assay....


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 155


References

Street et al., 2019 to be updated