Difference between revisions of "Part:BBa K3098008"

 
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<partinfo>BBa_K3098008 short</partinfo>
 
<partinfo>BBa_K3098008 short</partinfo>
  
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In order to purify the PgD5 produced by E.Coli and increase its solubility, a GST tag is added at the N-terminal of the sequence, GST tag can help the peptide dissolve in cytoplasm to help the expression of the peptide. During purification, GST can bind with the column , after adding the specific enzyme, GST tag can be cut to free the target peptide PgD5.
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In order to purify the PgD5 produced by E.Coli and increase its solubility, a GST tag is added at the N-terminal of the sequence, it can help the peptide dissolve in cytoplasm to help the expression of the peptide.  
  
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<!-- Add more about the biology of this part here
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<During purification, GST tag can bind with the corresponding column to keep the target protein when the impurities leave. After washing, PreScission Protease will be added to cut the GST tag from the target protein
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===Usage and Biology===
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Revision as of 11:25, 21 October 2019


GST+PgD5

In order to purify the PgD5 produced by E.Coli and increase its solubility, a GST tag is added at the N-terminal of the sequence, it can help the peptide dissolve in cytoplasm to help the expression of the peptide.

<During purification, GST tag can bind with the corresponding column to keep the target protein when the impurities leave. After washing, PreScission Protease will be added to cut the GST tag from the target protein

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 688
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 784
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85