Difference between revisions of "Part:BBa K3111032:Design"
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===Source=== | ===Source=== | ||
| − | + | MiniSOG was derived from Arabidopsis thaliana. | |
===References=== | ===References=== | ||
Revision as of 20:51, 30 September 2019
Superfolder GFP fused to T. maritima encapsulin loading peptide
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 697
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence was optimised for E. coli expression. Two illegal PstI sites removed from 44-Q and 95-Q (CAG to CAA). 15aa targeting peptide was chosen based on work by Cassidy-Amstutz et al, 2016.
Source
MiniSOG was derived from Arabidopsis thaliana.