Difference between revisions of "Part:BBa K3111032:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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15aa linker was chosen based on work y Cassidy-Amstutz et al.
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Sequence was optimised for E. coli expression. Two illegal PstI sites removed from 44-Q and 95-Q (CAG to CAA). 15aa targeting peptide was chosen based on work by Cassidy-Amstutz et al, 2016.
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===Source===
 
===Source===

Revision as of 20:50, 30 September 2019


Superfolder GFP fused to T. maritima encapsulin loading peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 697
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was optimised for E. coli expression. Two illegal PstI sites removed from 44-Q and 95-Q (CAG to CAA). 15aa targeting peptide was chosen based on work by Cassidy-Amstutz et al, 2016.

Source

Derived from Arabidopsis thaliana.

References