Difference between revisions of "Part:BBa J01003"

Revision as of 17:08, 30 September 2019

OriT-R (Origin of transfer for the R-plasmid nic region)

OriTR, the R plasmid nic region, is where the relaxosome nicks the plasmid and conjugative transfer by R plasmid machinery begins.

This oriT was characterized by Heidelberg iGEM2008 team (see experience). In this characterization the conjugation rate, conjugation time, conjugation efficiency between different strains, at different times and temperatures was measured. Furthermore the influence of donor : recipient ratio was analysed.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 117
Illegal NgoMIV site found at 127
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 19

@@ Line 29: / Line 29: @@
 As the figure indicates, the RP4-8 ensures an overall uniform transfer of both the donor plasmid, the mobilizing plasmid and both of the plasmids at once. The pRK24 mobilizing plasmid seemed to favor the transfer of the mobilizing plasmid rather than the donor plasmid, contributing to a less uniform transfer of the different plasmids. However, no significant differences between the two mobilizing plasmids was observed. <br>
-[1] [https://www.uniprot.org/uniprot/P0A7S3] <br>
+[1] [https://www.uniprot.org/uniprot/P0A7S3]
-[2] [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2293198/] <br>
+[2] [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2293198/]
-[3] [https://www.addgene.org/51950/] <br>
+[3] [https://www.addgene.org/51950/]
-[4] [https://www.addgene.org/79813/] <br>
+[4] [https://www.addgene.org/79813/]