Difference between revisions of "Part:BBa K3128033"
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Clickable OmpX is an outer membrane protein whose C and N terminals are in the intracellular domain. It can be used as a scaffold, a non-natural amino acid must be introduced. | Clickable OmpX is an outer membrane protein whose C and N terminals are in the intracellular domain. It can be used as a scaffold, a non-natural amino acid must be introduced. | ||
| − | This can be done through the presence of the stop codon TAG amber in the third loops OmpX.. With a specific tRNA, an azide functionalized amino acid can be integrated, which can be used for the SPAAC click chemistry reaction with DBCO / DIBO functional groups | + | This can be done through the presence of the stop codon TAG amber in the third loops OmpX.. With a specific tRNA, an azide functionalized amino acid can be integrated, which can be used for the SPAAC click chemistry reaction with DBCO / DIBO functional groups |
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Revision as of 18:50, 29 September 2019
COMP under constitutive promoter
Clickable OmpX is an outer membrane protein whose C and N terminals are in the intracellular domain. It can be used as a scaffold, a non-natural amino acid must be introduced. This can be done through the presence of the stop codon TAG amber in the third loops OmpX.. With a specific tRNA, an azide functionalized amino acid can be integrated, which can be used for the SPAAC click chemistry reaction with DBCO / DIBO functional groups
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]