Difference between revisions of "Part:BBa S05497"
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<partinfo>BBa_S05497 short</partinfo> | <partinfo>BBa_S05497 short</partinfo> | ||
− | Construction intermediate | + | Construction intermediate: |
+ | This biobrick is the result of the binding of a constitutive promoter (BBa_J23101) and an RBS (BBa_B0034) by 3AAssembly, | ||
+ | once linked to a CDS followed by a terminator, it will allow the normal expression of the gene | ||
+ | iGEM Grenoble alpes has designated this biobrick to bind it to the biobrick (BBa_K3128032) OmpX mutated at the level of tyrosine, itself linked to the terminator in order to be able to express a clickable OmpX protein | ||
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Latest revision as of 17:19, 29 September 2019
J23101:B0034
Construction intermediate: This biobrick is the result of the binding of a constitutive promoter (BBa_J23101) and an RBS (BBa_B0034) by 3AAssembly, once linked to a CDS followed by a terminator, it will allow the normal expression of the gene iGEM Grenoble alpes has designated this biobrick to bind it to the biobrick (BBa_K3128032) OmpX mutated at the level of tyrosine, itself linked to the terminator in order to be able to express a clickable OmpX protein
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]