Difference between revisions of "Part:BBa K112002:Design"

 
(→‎Design Notes)
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K112002 short</partinfo>
 
<partinfo>BBa_K112002 short</partinfo>
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===Design Notes===
 
===Design Notes===
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PCR bx1/bx4 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
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Sub into pBca1256 (EcoRI/BamHI)
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Product is pBca1256-K112002
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----------------------------------------
 +
bx1 Forward biobricking of {T4 Holin>}
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ccagtGAATTCatgAGATCTatggcagcacctagaatatc
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bx4 Reverse biobricking of {T4 Holin>}
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cgttaGGATCCtttagcccttcctaatattctggcc
  
 
===Source===
 
===Source===

Revision as of 23:28, 3 September 2008

T4 holin, without stop codon, berkeley standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR bx1/bx4 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112002

bx1 Forward biobricking of {T4 Holin>} ccagtGAATTCatgAGATCTatggcagcacctagaatatc bx4 Reverse biobricking of {T4 Holin>} cgttaGGATCCtttagcccttcctaatattctggcc

Source

References