Difference between revisions of "Part:BBa K2973016:Experience"

 
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===Applications of BBa_K2973016===
 
===Applications of BBa_K2973016===
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In our project, we used the IS6110 gene as a DNA template, in order to perform isothermal amplification reactions with Recombinase Polymerase Amplification (RPA). Using this method, we were able to detect quantities as low as 10 to the minus 9 nanograms in reaction times ranging from five to 20 minutes.
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RPA reaction
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<html>
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  <head>
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      <title>HTML img Tag</title>
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  </head>
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  <body>
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      <img src="https://static.igem.org/mediawiki/parts/3/31/Thessaly2019_RPA_IS6110_Gel.jpeg" width="251"
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        height="225">
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  </body>
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</html>
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PCR reaction
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<html>
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  <head>
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      <title>HTML img Tag</title>
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  </head>
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  <body>
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      <img src="https://static.igem.org/mediawiki/parts/e/e0/Thessaly_2019_PCR_IS6110_Gel.jpeg" width="285"
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        height="350">
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  </body>
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</html>
  
 
===User Reviews===
 
===User Reviews===

Revision as of 19:47, 28 September 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2973016

In our project, we used the IS6110 gene as a DNA template, in order to perform isothermal amplification reactions with Recombinase Polymerase Amplification (RPA). Using this method, we were able to detect quantities as low as 10 to the minus 9 nanograms in reaction times ranging from five to 20 minutes.

RPA reaction HTML img Tag

PCR reaction HTML img Tag

User Reviews

UNIQf2f6b093aa623999-partinfo-00000002-QINU UNIQf2f6b093aa623999-partinfo-00000003-QINU