Difference between revisions of "Part:BBa K2973009:Design"

Revision as of 20:18, 28 September 2019

IS6110 (Mycobacterium tuberculosis)

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 880
Illegal XhoI site found at 1266
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 232
Illegal NgoMIV site found at 1305
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1314
Illegal BsaI.rc site found at 1295

Design Notes

IS6110 has normally two open reading frames. OrfA (327 bp) and orfB (927 bp) are consecutive ORFs partially overlapping in the relative translational reading frames 0 and −1, respectively. For the purposes of our project, we designed primers for orfB only.

To have enough DNA material to work for our project, we cloned this part in pGEM-T via TA cloning.

Source

NCBI Mycobacterium tuberculosis Genome, Strain H37Rv, Accession Number: NC_000962

References

D Thierry, A Brisson-Noël, V Vincent-Lévy-Frébault, S Nguyen, J L Guesdon, B Gicquel, Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis (1990), Journal of Clinical Microbiology

Thabet, S., & Souissi, N. (2016). Transposition mechanism, molecular characterization and evolution of IS6110, the specific evolutionary marker of Mycobacterium tuberculosis complex. Molecular Biology Reports, 44(1), 25–34.doi:10.1007/s11033-016-4084-x

@@ Line 9: / Line 9: @@
 IS6110 has normally two open reading frames. OrfA (327 bp) and orfB (927 bp) are consecutive ORFs partially overlapping in the relative translational reading frames 0 and &#8722;1, respectively. For the purposes of our project, we designed primers for orfB only.
+To have enough DNA material to work for our project, we cloned this part in pGEM-T via TA cloning.
 ===Source===