Difference between revisions of "Part:BBa K2973014"

Revision as of 16:31, 28 September 2019

Trigger for Toehold switch 14 Dictyoglomus turgidum Toehold switch systems are composed of two RNA strands referred to as the switch and trigger. Upstream of the coding sequence is a hairpin-based processing module containing both a strong RBS and a start codon that is followed by a common 21 nt linker sequence coding for low-molecular-weight amino acids added to the N terminus of the gene of interest. A single-stranded toehold sequence at the 5’ end of the hairpin module provides the initial binding site for the trigger RNA strand. This trigger molecule contains an extended single stranded region (36bp) that completes a branch migration process with the hairpin to expose the RBS and start codon, thereby initiating translation of the coding gene. This trigger sequence was derived from the 16S rRNA sequence of Dictyoglomus turgidum.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 64
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K2973014 short</partinfo>
+Toehold switch systems are composed of two RNA strands referred to as the switch and trigger. Upstream of the coding sequence is a hairpin-based processing module containing both a strong RBS and a start codon that is followed by a common 21 nt linker sequence coding for low-molecular-weight amino acids added to the N terminus of the gene of interest. A single-stranded toehold sequence at the 5’ end of the hairpin module provides the initial binding site for the trigger RNA strand. This trigger molecule contains an extended single stranded region (36bp) that completes a branch migration process with the hairpin to expose the RBS and start codon, thereby initiating translation of the coding gene.
-Sa
+This trigger sequence was derived from the 16S rRNA sequence of Dictyoglomus turgidum.
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