Difference between revisions of "Part:BBa K2938006"

 
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Cry4Ba Bti toxin with HA-tag attached in the N- terminal in order to be able to detect the translated protein in western blot (using antibodies against the tag)
 
Cry4Ba Bti toxin with HA-tag attached in the N- terminal in order to be able to detect the translated protein in western blot (using antibodies against the tag)
  
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===Usage and Biology===
 
===Usage and Biology===
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The Cry4Ba is a toxin with larvicidal effect on mosquitos’ larvae, and HA-tag is used to detect proteins in western blot using antibodies specific to the tag.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 10:46, 28 September 2019


Cry4Ba + HA tag

Cry4Ba Bti toxin with HA-tag attached in the N- terminal in order to be able to detect the translated protein in western blot (using antibodies against the tag)

Usage and Biology

The Cry4Ba is a toxin with larvicidal effect on mosquitos’ larvae, and HA-tag is used to detect proteins in western blot using antibodies specific to the tag. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1708
    Illegal SpeI site found at 2397
    Illegal PstI site found at 212
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 96
    Illegal SpeI site found at 2397
    Illegal PstI site found at 212
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1708
    Illegal SpeI site found at 2397
    Illegal PstI site found at 212
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1708
    Illegal SpeI site found at 2397
    Illegal PstI site found at 212
    Illegal NgoMIV site found at 2520
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 502