Difference between revisions of "Part:BBa K2938005"
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Cry11Aa Bti toxin with Strep-tag attached in the N- terminal in order to be able to detect the translated protein in western blot (using antibodies against the tag)
 
Cry11Aa Bti toxin with Strep-tag attached in the N- terminal in order to be able to detect the translated protein in western blot (using antibodies against the tag)
  
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===Usage and Biology===
 
===Usage and Biology===
 
The Cry11Aa is a toxin with larvicidal effect on mosquitos’ larvae, and strep-tag is used to detect proteins in western blot using antibodies specific to the tag.
 
The Cry11Aa is a toxin with larvicidal effect on mosquitos’ larvae, and strep-tag is used to detect proteins in western blot using antibodies specific to the tag.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 10:43, 28 September 2019


Cry11Aa + Strep tag

Cry11Aa Bti toxin with Strep-tag attached in the N- terminal in order to be able to detect the translated protein in western blot (using antibodies against the tag)


Usage and Biology

The Cry11Aa is a toxin with larvicidal effect on mosquitos’ larvae, and strep-tag is used to detect proteins in western blot using antibodies specific to the tag.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1719
    Illegal SpeI site found at 831
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1719
    Illegal SpeI site found at 831
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1719
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1719
    Illegal SpeI site found at 831
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1719
    Illegal SpeI site found at 831
  • 1000
    COMPATIBLE WITH RFC[1000]